Vine Brook Bikeway is a hiking and biking trail in Lexington, Massachusetts. It is within Lower Vine Brook. It is 0.6 miles long and begins at 183 feet altitude. Traveling the entire trail is 1.3 miles with a total elevation gain of 26 feet. The Lower Vine Brook conservation is near the trailhead. The Lower Vine Brook conservation and the American Beech tree can be seen along the trail.