Horseshoe Bluff Nature Trail is a hiking trail in Dubuque County, Iowa. It is within Mines of Spain State Recreation Area. It is a mile long and begins at 624 feet altitude. Traveling the entire trail is two miles with a total elevation gain of 413 feet. The Horseshoe Bluff Nature Trailhead parking is near the trailhead. There are also restrooms and a shelter. The Ampitheater attraction and Horseshoe Bluff (elevation 771 feet) and other cliffs can be seen along the trail. The trail ends near the Observation Deck viewpoint.